Antioxidant and nematicidal properties were evaluated for R. emodi extractives which are extracted by standardizing and adopting accelerated solvent extraction (ASE) method along with traditional Soxhlet extraction. The extracted material was separated using flash chromatography and the separation conditions and solvents were standardized for the extracted plant constituents. The purity was detected by using analytical reverse phase high pressure liquid chromatography (HPLC). LC-MS/MS detection in the direct infusion mode of the isolated, purified products afforded four anthraquinones, characterized by their infrared spectra (IR) and 1H spectra as chrysophanol, physcion, emodin, and aloe-emodin. Five antraquinone glucoside derivatives and piceatannol-3-O-β-d-glucopyranoside have also been detected from the extracted product. During in vitro evaluation the antioxidant potential of methanolic crude extract (CE1) was the highest, followed by ethyl acetate crude extract (CE2) and chloroform extract (CE3) in DPPH radical scavenging activity. The CE1 also demonstrated outstanding nematicidal activity as compared with other extracts, pure anthraquinones, and even positive control azadirachtin. The study conclusively demonstrated the antioxidant potential of R. emodi extracts and also its ability in extenuating the Meloidogyne incognita (root-knot nematode). The bioassay results can be extrapolated to actual field condition and clinical studies.