Cracks caused by environmental temperature and humidity variation are generally considered one of the most important factors causing durability deterioration of concrete structures. The seasonal or daily variation of ambient temperature and humidity can be considered periodic. The dynamic modulus of elasticity is an important parameter used to evaluate the performance of structural concrete under periodic loads. Hence, in this paper, the dynamic elastic modulus test of concrete under simulating periodic temperature-humidity variation is carried out according to monthly meteorological data of representative areas (Nanjing, China). The dynamic elastic modulus attenuation pattern and a dynamic elastic modulus degradation model of concrete under periodic temperature-humidity are investigated. The test results show that the dynamic elastic modulus of concrete decreases and tends to be stable under the action of periodic temperature-humidity. Comparative analysis shows that the two-parameter dynamic elastic modulus degradation model is more suitable for describing the dynamic elastic modulus attenuation pattern of concrete under periodic temperature-humidity action than the single-parameter one.