Twelve Ayrshire cows were used to study the effects of and interactions between extent of silage fermentation and level of protein supplementation on silage intake and milk production. Experimental design was a cyclic change-over with six dietary treatments, with two replicate blocks of six cows and four 3-week periods. Dietary treatments in a 2 x 3 factorial arrangement consisted of two wilted silages treated with either a formic acid-based (FA) 5 l/t or a bacterial inoculant additive (I) and three levels of protein supplementation obtained by fish meal (FM) inclusion (0, 60 and 120 g/kg concentrate). Grass silage was given ad libitum and concentrates were offered at a rate of 10 kg/d. Both silages were well-preserved but I-silage was more extensively fermented than FA-silage. Feeding FA-silage decreased diet organic matter digestibility, particularly that of neutral detergent fibre, compared with I-silage. Inclusion of FM increased the digestibility of all dietary constituents. Dry matter (DM) intake of I-silage was lower than that of FA-silage but milk yield was not significantly affected. Restricting silage fermentation increased milk fat content, fat yield and energy corrected milk yield. FM inclusion increased silage DM intake, milk yield, milk protein content and yield. FM120 inclusion increased milk fat yield more with FA-silage than with I-silage, whereas the response in milk protein yield was greater with I-silage. Plasma glucose, plasma urea and milk urea concentrations were lower and blood β-hydroxybutyrate higher in cows given FA-diets than those given I-diets.