The aim of the research was to investigate the effect of positive thinking training on psychological well-being and perceived quality of marital relationship on infertile women. The research method was quasi-experimental with pretest- posttest and 3 months follow up, with a control group. Among the population of infertile women, twenty infertile women (10 persons in experimental group, 10 persons in control group) were selected by purposeful sampling method and were assigned in experimental and control groups. The experimental group received 8 session (1 ½ hours for each) of positive thinking training. For data collection, Ryff Psychological Well-Being Questionnaire and Fletcher Perceived Quality of Marital Relationship Scale were used. Multivariate analysis of covariance (MANCOVA) was used for analyzing the data. The results showed the positive thinking training was effective on the psychological well-being and quality of marital relationship among infertile women. Also, the training was effective in a 3 months follow up. Therefore, positive thinking skills can be used in prevention actions.