Aim: To evaluate the efficacy of commercially available nano-hydroxyapaptite paste as a desensitizing agent. Materials & Methods: The study included 30 subjects complaining of dentinal hypersensitivity. After prophylactic scaling & root planing, patients were instructed to brush with the given commercially available nano-hydroxyapatite paste twice a day. A numeric rating scale was used to measure the parameter of pain related to the stimuli at the baseline and after the application of gel at the intervals of 24 hours, 1 month, 3 months and 6 months. Results: There was remarkable decrease in the dentinal hypersensitivity at the end of 6 months. Intragroup comparisons at various time intervals were significant (p<0.0001). The mean pain score at the end of 6 months was 3.00 +/- 0.468 from 7.93 +/- 0.248 at the baseline. Conclusion: Hence, commercially available nano-hydroxyapatite paste is effective in reducing dentinal hypersensitivity.