Activation of plant defense pathways can be influenced by the presence of different species of attacking organisms. Understanding the complicated interactions triggering plant defense mechanisms is of great interest as it may allow the development of more effective and sustainable disease control methods. and anastomosis group (AG) 2-1 are two important organisms attacking oilseed rape (OSR), causing disease and reduced yields. At present, is unclear how these two interact with each other and with OSR defenses and therefore the aim of the present study was to gain a better insight into the indirect interaction between aphids and pathogen. In separate experiments, we assessed the effect of AG 2-1 infection on aphid performance, measured as growth rate and population increase and then the effect of aphid infestation on AG 2-1 by quantifying disease and the amount of fungal DNA in plant stems and compost for two OSR varieties, "Canard" and "Temple." Additionally, we examined the expression of genes related to jasmonic acid (JA) and salicylic acid (SA) defense pathways. There was no significant effect of AG 2-1 infection on performance. However, aphid infestation in one of the varieties, "Canard," resulted in significantly increased disease symptoms caused by AG 2-1, although, the amount of fungal DNA was not significantly different between treatments. This meant that "Canard" plants had become more susceptible to the disease. Expression of and was elevated under AG 2-1 treatment but downregulated in plants with both aphids and pathogen. Therefore it seems plausible that alterations in the JA signaling due to aphid infestation resulted in the increased susceptibility to AG 2-1.