uso de scanner em espectrofotometria de absorção molecular: aplicação em experimento didático enfocando a determinação de ácido ascórbico molecular absorption spectrophotometry using a scanner: proposal of a didactic experiment for ascorbic acid determination
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uso de scanner em espectrofotometria de absorção molecular: aplicação em experimento didático enfocando a determinação de ácido ascórbico molecular absorption spectrophotometry using a scanner: proposal of a didactic experiment for ascorbic acid determination

;Marcos S. Gomes;Lilian C. Trevizan;Joaquim A. Nóbrega;Marcos Y. Kamogawa
greece and rome 2008 Vol. 31 pp. 1577-1581
251
gomes2008qumicauso

Abstract

Quantitative analysis of colored substances is generally performed using a spectrophotometer for detection of radiation. However, the combination of digital images and mathematical conversion of colors is an alternative for quantitative procedures based on colored chemical reactions. This work proposes a didactic experiment using a scanner for determination of ascorbic acid in medicines based on the formation of the Prussian blue complex. Results were in good agreement with a conventional spectrophotometer method and the procedure is attractive for instrumental analysis undergraduate courses.

Keywords

spectrophotometry ascorbic acidchemistry

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DOI:
10.1590/S0100-40422008000600050
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