Objective: To study the value of serum procalcitonin content for assessing the inflammation and organ injury in neonatal septicemia. Methods: 48 children with neonatal septicemia who were treated in our hospital between April 2014 and May 2016 were selected as the observation group, 50 healthy newborns who were delivered in our hospital during the same period were selected as the normal control group, and the observation group were further divided into high PCT group and low PCT group (n=24) according to the median of serum PCT content. Enzyme-linked immunosorbent assay (ELISA) was used to detect the serum contents of inflammatory mediators, and color Doppler diasonograph was used to measure heart injury index levels. Results: Peripheral blood PCT content of observation group was significantly higher than that of control group (P<0.05); serum inflammatory mediators IL-1β, IL-6, IL-8 and TNF-α contents of high PCT group and low PCT group were significantly higher than those of normal control group, and as the PCT content increased, serum inflammatory mediators interleukin-1β (IL-1β), interleukin-6 (IL-6), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) contents increased (P<0.05); routine ultrasound parameters cardiac output (CO) and left ventricular ejection fraction (LVEF) levels as well as the absolute value of twodimensional speckle tracking imaging parameters left ventricular global longitudinal strain rate (GLSr) and left ventricular global circumferential strain rate (GCSr) of high PCT group and low PCT group were lower than those of normal control group while serum myocardial injury indexes cardiac troponin I (cTnI), heart-type fatty acid-binding protein (H-FABP) and α-hydroxybutyric dehydrogenase (HBDH) contents were higher than those of normal control group, and as the PCT content increased, CO and LVEF levels as well as the absolute value of GLSr and GCSr decreased while the indexes cTnI, H-FABP and HBDH contents increased (P<0.05). Conclusion: Serum PCT content is positively correlated with the degree of inflammation and heart injury in neonatal septicemia, and can be used as a reliability index to early judge the disease severity and the target organ damage.