A new species of Trichothurgus, described herein, T. bolitophilus sp. n., nests in dry horse manure pads in Chubut, Patagonia, Argentina. The simplest nests consisted of one main tunnel ending in a series of 2 cells without partitions between them. In the more complex ones up to 6 cells were connected laterally to the main tunnel. Nests showed signs of reutilization. The behavior of nesting in horse manure is described for the first time in bees.