Metabolic profile is used to detect possible nutritional errors with negative effects on animals production. The aim of this study was to establish some reference values of blood constituents for lactating buffaloes related to the season. Thus, 15 buffalo females, clinically healthy, from the S.C.D.C.B. Sercaia-Brasov were screened for hematological and biochemical blood profile during the summer and the winter period. Feeding system was differentiated by season (conserved forage and corn fodder in the winter season and green mass in the summer season). The results for hematological parameters revealed no significantly statistical differences (p>0.05) between seasons. The average values of the determined biochemical parameters (total cholesterol, serum levels of Mg) had values within the normal range, with no statistically differences between seasons. Total protein level varied significantly distinct (p<0.01) between the winter season 7.02±1.13 mg/dL and the summer season 8.20±0.92 mg/dL.  The values obtained for the mineral profile (serum Ca and inorganic P) varied significantly (p<0.05) between seasons. The level of PAL and GOT enzymes registered values above normal physiological limits, the activity of these enzymes being considered as an indicator for acute liver necrosis. The obtained values in this study could be useful for interpretation of biochemical and hematological parameters in lactating buffaloes.