Mesoporous structured molecularly imprinted polymers combined with restricted access materials (RAM-M-MIPs) were synthesized in this work, then RAM-M-MIPs were used as adsorbents in matrix solid phase dispersion (MSPD) to extract chlorpyrifos residues from soil. The RAM-M-MIPs with mesoporous structure increased adsorptive capacity and accelerated adsorption rate. At the same time, the RAM-M-MIPs with restricted access function showed good exclusion effect toward humic acid in the soil. In addition, the characteristics of RAM-M-MIPs were measured and the optimal experimental conditions of MSPD were explored. In MSPD, the extraction and clean-up procedures can be finished simultaneously, which saved the extraction time. The analyte eluted from soil was determined by LC-MS/MS. The recoveries were in the range of 93.5-106.1% and the relative standard deviations were in the range of 2.8-5.7% at the spiked soil levels of 0.10-10.00 ng g, the detection limit was 0.015 ng g. Based on these analytical validation results, the developed method can be a useful method to determine chlorpyrifos in soil.