an integration-free, virus-free rhesus macaque induced pluripotent stem cell line (ripsc90) from embryonic fibroblasts
;Enrique Sosa;Rachel Kim;Ernesto J. Rojas;Linzi Hosohama;Jon D. Hennebold;Kyle E. Orwig;Amander T. Clark
journal of energy chemistry2017Vol. 21pp. 5-8
176
sosa2017steman
Abstract
The rhesus macaque induced pluripotent stem cell (riPSC) line, UCLAi090-A (riPSC90), was generated from rhesus embryonic fibroblast (REF) cells called REF90. REF90 cells and the riPSC90 line were authenticated by short tandem repeat analysis and had a normal male (42, XY) karyotype. The riPSC90 line expressed markers of self-renewal including OCT4, NANOG, TRA-1-81 and SSEA4, and generated teratomas after transplantation into immunocompromised mice. riPSC90 could be used in parallel with riPSC89, which was derived from REFs cultured from a different rhesus macaque embryo (Sosa et al. 2016).