The present study was designed to analyse the personality structure of children aged one to seven, as perceived by their kindergarten teachers. In addition, gender differences were examined to determine whether kindergarten teachers perceived the personality characteristics of toddler and pre-school girls differently than those of boys. 508 randomly-selected Slovenian children were assessed by their kindergarten teachers using adapted Flemish Big-Five Bipolar Rating Scales. Four-factor structures that explained more than two-thirds of the variance emerged for teachers' personality ratings of children in each of the three age groups: toddlers, three- to five-year-olds and five- to seven-year-olds. However, five of the twenty-five scales, four of them referring to the Conscientiousness dimension, did not appear to be relevant when assessing individual differences in the toddlerhood. Intellect/Openness, as observed for the toddler sample, and the combined Conscientiousness-Intellect/Openness factor, extracted for each of the two older age groups, accounted for the greatest part of the variance. Extroversion was obtained as a second factor in each of the three age groups, while Emotional Stability showed relatively less stability across these groups. Agreeableness was clearly differentiated only in the oldest age group, emerging there for the first time as an independent factor. A few gender differences were found to be significant within the two groups of pre-school children, with girls consistently being rated higher in terms of Conscientiousness-Intellect/Openness.