Background: Cutaneous leishmaniasis (CL) is a major health problem in many parts of Iran, although diagnosis of CL especially in the endemic area is easy, but treatment and management of the disease is a global dilemma. Diagno­sis of CL in non-endemic area is not as simple as in endemic foci. In this study, the status and the proportions of CL induced by Leishmania major and L. tropica among CL suspected patients referred to the Center for Research and Training in Skin Diseases and Leprosy, (CRTSDL) during 2008 to 2011 are described.Methods: CL patients with suspected lesions were clinically examined. History of trip to zoonotic CL and/or anthroponotic CL endemic areas and the characte­ristics of their lesion(s) were recorded. Diagnosis of the lesion was done using direct smear microscopy, culture and conventional polymerase chain reaction (PCR).Results: A total of 404 (M=256, F=148) patients with 776 lesions were re­cruited and parasitologically examined. The results showed that 255 of the pa­tients with 613 lesions; patients with lesion(s) induced by L. major=147 (M=63, 43%, F=84, 57%) and lesion(s) induced by L. tropica=108 (M=35, 32%, F=73, 68%). History of travel to endemic area was not always correlated with isolated Leishmania species.Conclusion: Although travel history to endemic area is an important factor to be considered for diagnosis, but parasitological confirmation is necessary initia­tion of treatment.