A quantitative real time PCR assay to detect and enumerate Escherichia coli O157 and O26 serogroups in sheep recto-anal swabs.
Abstract
A quantitative PCR method is described for the detection and quantification of E. coli O157 and O26 in sheep recto-anal junction swabs. The method incorporated a short enrichment step (5 h) and the use of a developed standard calibration curve relating the real time PCR cycle threshold (Ct) values against the initial concentration of pathogen in the sheep sample.
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20673
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0x95644003c57E6F55A65596E3D9Eac6813e3566dA
Article ID:
20673
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S0167-7012(19)30567-6
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Scimatic Chain (ID: 481)
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