Palaeo-, rock magnetic and geochemical studies were conducted on volcanic samples from the Lištice area to improve the knowledge of Palaeozoic volcanic evolution in the Prague Basin. The magnetic data display no significant differences between two studied localities, indicating one magnetizing event for both localities. Geochemical data suggest that Lištice basalt could have originated from deep melting of the garnet peridotite mantle source during the attenuation and rifting of the continental lithosphere connected with asthenospheric mantle upwelling. The dataset furthermore supports the evidence of syn- or post-intrusive fluid interactions and low-temperature stages of alteration. The Ti-magnetite within amygdales of the samples was found to be carrying the characteristic remanent magnetization and reflects probably the Permo-Carboniferous remagnetization of volcanic phases.