Background & Objectives: Studying periods in university is very important for students. Because of the problems, this period is usually accompanied with mental status changes of students. The aim of this study was the assessment of psychological variables (stress, anxiety and depression) and self-concept of students. Materials & Methods: In this cross-sectional study, all the students studying at Fasa University of Medical Sciences in the academic year 89-88 were selected through census sampling method. The DASS-21 was used to assess stress, anxiety and depression of students and in order to evaluate the status of their self-concept; the Carl Rogers questionnaire was used. Data analysis was performed with SPSS software using descriptive and inferential statistics (t test, ANOVA, Chi square and Pearson correlation). Results: The results of this study showed that 76% of students had stress, 56.4% anxiety and 53.1% depression, and 69/3% had weak or negative self-concepts. There was a statistically significant correlation between high stress, anxiety and depression with negative self-concept (P<0.001).Conclusion: High stress, anxiety and depression and also a significant correlation between increased stress, anxiety and depression with negative and weak self-concept of students were found. It is necessary to devote more careful attention to mental health issues of students and have appropriate interventions.