Objective: To study the expression of PPARγ and AQP7 in 3T3-L1 adipocyte differentiation and the promoting effect of emodin. Methods: In the process of adipocyte differentiation induced by traditional adipocyte differentiation inducer, different concentration of emodin was added for co-incubation, and the changes in the expression of adipose differentiationrelated marker genes FAS, AP2, GR, LPL and Pref-1 as well as the PPARγ and AQP7 protein expression were detected. Results: The study confirmed that during 3T3-L1 adipocyte differentiation process, differentiation marker proteins FAS, AP2, GR, LPL and Pref-1 expression significantly increased, AQP7 and PPARγ expression increased, and emodin had dose-dependent promoting effect on cell differentiation. Conclusion: emodin has promoting effect on the process of 3T3-L1 adipocyte differentiation, and this process may be related to PPARγ and AQP7 expression regulated by it.