Synthesis of silver nanoparticles (AgNPs) through biological route plays an important role in their applications in the medical field, especially in the prevention of disease causing microbial pathogens and arresting the propagation of cancer cells. The stable, green synthesis of AgNPs is very much welcomed in the medical field because of their low toxicity. Therefore, the demands of AgNPs synthesised biologically is on the rise. The present study aimed to investigate the antimicrobial mechanisms and anticancer properties of the AgNPs synthesized using the seed extract of L. The AgNPs were characterized by UV-vis, SEM, XRD, FTIR and EDAX analysis. The minimum inhibitory concentrations (MIC) of the AgNPs were determined by the broth micro dilution method.The formation of brownish red color indicated the formation NPs with the absorption maximum at 420 nm. The average size was found to be 33.93 nm and sphere shaped. The FTIR spectrum revealed the absorption bands at 3340 cm and 1635 cm indicated the presence of -OH or -COOH and amide group stretching in the AgNPs. The X-ray diffraction report confirmed the presence of strong peak values of 2θ within the angle of 37.1°. The lowest MIC of the AgNPs against was 62.5 μg mL. MIC values against and were 125 and 250 μg mL respectively. The MIC of the AgNPs against , and were each 250 μg mL, respectively. The extracellular protein concentration, levels of lactate dehydrogenase and alkaline phosphtase enzyme in the AgNPs treated bacterial pathogens demonstrated greater antimicrobial mechanism. Additionally, the AgNPs exhibited significant anticancer activity against the MCF7 and Vero cell lines.The synthesized AgNPs could be further evaluated in large scale as a botanical antimicrobial agent.