The idea of using chemical agents in removal of obstructions on the walls of root canals has been existing since years ago. Successful attempts in this area have been made with the introduction of RC-Prep materials in root canal preparations. The aim of this study was to compare the decalcifing potency of two chelating agents in root canal preparations. 30 extracted canines were divided in three groups for use of Re- Prep materials. Group A Rc-Prep (Primer) made in USA, group B Re- Prep (RC-CTF) made in Iran, and group C as control. Root canals were prepared using standard method followed by washing with 5% sodium hypochloride. Then the teeth were divided into two sections of mesial and distal by a special cutting device. The amount of calcium on polished sections was measured using an elemental analysis method (electron beam probe system). The mean value data were analysed using one¬way Analysis of Variance (ANOVA). The mean values for the measured calcium were group A: 28713±1129, group B: 39441+680, and group C: 54458±2024 . Statistical analysis showed that the mean value for group A was significantly different from other groups. There was also significant difference in the mean values for group B and C. The results indicate that the decalcifing potency for Rc-Prep (Primer, USA) is significantly higher (PO.001) than that of Rc-Prep (RC-CTF).