Due to the ever-increasing of antibiotic-resistance microorganisms and the tendency towards the application of natural preservatives, in the present study the ethanolic extract of stalk portion of  Kurdistan Gundelia tournefertti L. was extracted in rotary evaporator. The antibacterial effect (MIC and MBC) of the extract was investigated against Staphylococcus aureus and Escherichia coli. using agar dilution assay on Muller-Hinton agar. The experiment was conducted with 3 replicates and probity analysis of the data was analyzed using SAS 9.2 software.Result showed that both MIC and MBC for S. aureus was62.5 µg/ml. Moreover, the MBC and MIC values for E. coli were estimated at 31.25 µg/ml and 15.62 µg/ml, respectively.Since ethanolic extract of G. tournefertti was highly effectiveon indicator bacteria,it can be used in combination with the other preservatives to protect foods from foodborne organisms.