Introduction: Given the importance of using e-learning in medical education, this study aimed to Anticipate the e-learning acceptance according to  nursing students enthusiasm scale at Gonabad University of Medical Sciences. Methods: This cross sectional study conducted on all undergraduate students of nursing and midwifery who were enrolled at Gonabad University of Medical Sciences in 2015. 172 students were recruited and enthusiasm and e-learning questionnaire were filled for them. SPSS V.16 package and the independent t-test, Pearson correlation and multiple regression model were used to analyse data. Results: The results showed there is no significant differenceهد  the relation between male and female students' enthusiasm(P>0.05). There is a significant relationship between academic enthusiasm component with e-learning acceptance (p<0.01), and components of the behavioral, emotional and cognitive enthusiasm, with  acceptance of e-learning. Conclusion: The findings showed that the components of academic enthusiasm is predicting acceptance of e-learning by students. Workshops and training courses may enhance students' enthusiasm. Stakeholders should also be organized by universities to enhance acceptance of e-learning.