Stem-end rot caused by is a destructive disease of mango. field isolates resistant to carbendazim (MBC) were collected in Hainan Province, China. In this study, the characteristics of these field isolates with resistance to MBC were investigated. The resistance of isolates to MBC was stably inherited. Both the MBC-resistant and MBC-sensitive isolates had similar mycelial growth rates, pathogenicity, sensitivity to high glucose, glycerol content, and peroxidase activity. Compared with MBC-sensitive isolates, MBC-resistant isolates were more sensitive to low temperature and had a significant decrease in sensitivity to high NaCl and a significant increase in catalase (CAT) and glutathione S-transferase GST) activities. After MBC treatment, the cell membrane permeability of the sensitive isolates was markedly increased compared with that of the resistant isolates. Analysis of the β-tubulin gene sequence revealed point mutations resulting in substitutions at codon 198 from glutamic acid (GAG) to alanine (GCG) in moderately resistant isolates, and at codon 200 from phenylalanine (TTC) to tyrosine (TAC) in highly resistant isolates. These β-tubulin gene mutations were consistently associated with MBC resistance. Overall, we infer that the altered cell membrane permeability and the increase in CAT and GST activities of the resistant isolates are linked to MBC resistance.