This study was conducted to determine the concentration of Vanadium (V) and Nickel (Ni) in the edible muscle tissues in male and female Fenneropenaeusindicus. For determination of the concentration of heavy metals in muscle tissues of edible Indian prawn in Shiraz city in autumn of 2011, 120 samples have been obtained randomly from Shiraz retail. Preparation and analysis of samples were performed by standard method using Atomic Absorption (Varian V10-ES) and then the concentrations of the heavy metals were compared with the recommended international standard. In this study the mean concentration of V in muscle of male and female were estimated at 1.06 ± 0.64 and 0.76 ± 0.54  mgKg-1, respectively. However, the concentration of Ni in muscle of male and female was determined as 8.1 ± 1.173 and 9.1 ± 0.79 mgKg-1, respectively. The analysis of the data showed that the concentration of Ni in the muscle of male and female samples had significant difference (P