The objective of this research was to evaluate the cutting preference of Acromyrmex laticeps nigrosetosus workers among three species and a Clone of eucalypt. The seedlings of Eucalyptus spp. were offered simultaneously to three colonies of this leaf-cutting ant under laboratory conditions. The assay ended six hours after initial offering or until the workers of a colony cut all leaves of a certain eucalyptus species. On the average, colonies cut and carried 0.453g ± 0.064 of Eucalyptus camaldulensis; 0.384g ± 0.052 of Clone 129; 0.341g ± 0.054 of Eucalyptus urophylla and 0.102g ± 0.027 of Eucalyptus cloeziana. This last species was the least preferred for cutting and carrying. On the other hand, there was no significant difference among the amount of leaves cut from the other eucalypts.