Chlorophyll is an important factor which also affects the yield in maize. In this study, genetic analysis of chlorophyll content was conducted by joint segregation analysis of four generations P1, P2, F1 and F2:4 from the cross Shen3336ıShen3265 using the mixed major genes and polygenes inheritance models. Genetics of chlorophyll revealed that chlorophyll “a” was controlled by two main gene having additive-dominanceepistasis effects. The heritability of these genes were 56.3%. Chlorophyll “b” was controlled by two pairs of codominant major gene plus additive-dominance polygene. The heritability of these major genes and polygenes were 1.12% and 93.26% respectively. Chlorophyll “a+b” was controlled by two pairs of additive-dominance- epistatic major genes plus additive-dominance polygene having heritability of 56.2% and 5.2% respectively.