A simple and rapid microextraction procedure based on dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-flame ionization detection (GC-FID) was developed for the extraction and analysis of 2-phenylethanol in rose water sample. In the proposed approach, carbon tetrachloride and ethanol were used as extraction and dispersive solvents, respectively. Some important parameters, such as extraction and disperser solvent and volume of them, extraction time, pH and salt effect were investigated. Under optimized conditions, a linear relationship was obtained in the range of 1.0-300.0 mg l-1. The method detection limit was 0.1 mg l-1. The relative standard deviations for the analysis of 2-phenylethanol were in the range of 1.5-2.4%. The relative recoveries of 2-phenylethanol at spiking levels of 10, 75 and 150 mg l-1 were 93.7, 96.9 and 97.2%, respectively. The enrichment factor of the proposed method was 123. The proposed method is a simple, fast, accurate, highly stable and selective and was applied for determination of 2-phenylethanol in rose water sample with satisfactory results.