Objective: To investigate the effects of Xuebijing injection combined with ulinastatin on endotoxin and inflammatory factors in treatment of severe acute pancreatitis. Methods: A total of 52 patients with severe acute pancreatitis in our hospital from July 2014 to July 2015 were selected and divided into two groups: Group A (n=26) and Group B (n=26). All of the patients received conventional treatment and necessary nutrition support. Patients of Group A were treated with ulinastatin on the basis of conventional treatment and nutrition support. Patients of Group B were treated with Xuebijing injection on the basis of Group A. Before and after treatment, the levels of serum endotoxin, hs-CRP, IL-6, IL-8, IL-10 and TNF-毩 were detected. Results: After treatment, the levels of hs-CRP and endotoxin in two groups were lower than before treatment, and the Group B decreased more significantly than the Group A. The levels of IL-6, IL-8 and TNF-毩 in two groups were lower than before treatment, and the Group B decreased more than the Group A, while levels of IL-10 increased more than before treatment, and the Group B increased more than the Group A. Conclusion: Xuebijing injection combined with ulinastatin can significantly improve the clinical treatment effect through reducing serum endotoxin levels and inhibiting the release of inflammatory factors of patients with severe acute pancreatitis.