This publication makes a faunistic contribution to knowledge of the epigeic spider species composition in the limestone quarry Malá Dohoda which is located in Moravian Karst PLA. The spiders were collected by pitfall trapping between catching period 19. 3.–1. 11. 2012. We chose 6 plots all around the quarry – in the inner part, edges of quarry and outside part of quarry. A total of 1 474 adult specimens were collected and determined as 78 species of 21 families. The most significant finding is vulnerable species (VU) Parapelecopsis nemoralis (Blackwall, 1841) which is the second record for Moravia. Among the other significant findings, both in term of rarity and bioindication, were Ceratinella major Kulczynski, 1894, Micaria formicaria (Sundevall, 1831), Ozyptila claveata (Walckenaer, 1837), Pelecopsis parallela (Wider, 1834), Zelotes longipes (L. Koch, 1866) and Zodarion rubidum Simon, 1914. Also, we present finding of an invasive species Mermessus trilobatus (Emerton, 1882) which is spreading in the Czech Republic since 2007. Together, we found 13 species as a novelty for studied faunistic square 6666 and we extended knowledge about new recorded species for the area of the Moravian Karst.