The standard technology of seed processing uses mainly chemical products. Recent researches showed that toxic materials from chemical fertilizers can be harmful to humans, animals and the environment. Currently the attention of researches is shifting away from chemical fertlizers and toward alternative that consumers perceive to be natural, Plant Growth Promoting bacteria (PGP). PGP bacteria could be a way to reduce chemical fertilizer doses. This was the reason to test the ability of Bacillus megaterium, Azotobacter chroococcum to produce hormone auxin (IAA). Bacterial strains were identified by PCR amplification and sequencing of the 16S rRNA gene. Indole-3-acetic acid (IAA) was detected and quantified by MRM experiment. This study conducted that maize seed inoculation with IAA from species mentioned above showed positive effects. They had statistically significantly higher root and steam height compared to control seedlings. Bacterial strains tested in this study may be recommended as PGP (Plant Growth Promoting) bacteria, due to their positive effects and eventually can be used to reduce chemical fertilizers doses.