Tetracycline (TC) antibiotics are widely used in livestock and poultry breeding. However, limited work has been done on the partition of TCs between suspended sediment (SPS) and overlying water or on the seasonal effects of rainfall events on the behavior of TCs in receiving rivers. Here, we assessed the impacts of rainfall events in different seasons on the concentrations and fate of TCs in a typical watershed. Concentrations of TCs in river water, SPS, and surface sediment were determined before, during, and after rainfall events. Results indicated that the sequence of TC concentration levels in river water was wet season > normal season > dry season. Rainfall events in all seasons increased the concentrations of TCs in river water. The concentration of TCs in SPS reached 104 ng/g. The SPS concentrations were only 22-78 mg/L, while the daily fluxes of TCs in particulate form contributed 39%-62% of the total (dissolved and particulate) daily fluxes in river water. The increases in TCs in river water were mainly attributed to internal release from sediment during rainfall events in the dry season but to external input during rainfall events in the wet season. The degradation products of TCs with higher concentrations and greater toxicity than their parent compounds should be considered in the ecological risk assessment of TCs. This research demonstrated that manure application should not be conducted in the normal season or before rainfall events, especially heavy rainfall.