This paper presents a singular prey-predator fishery model, where maturation delay for prey and gestation delay for predator are considered. Fishing efforts are introduced to harvest prey and predator population, which are developed as control instruments to investigate optimal utilization of fishery resource. By analyzing associated characteristic equation, local stability analysis is studied due to combined variations of double time delays. Furthermore, Pontryagin’s maximum principle is utilized to characterize optimal harvest control, and the optimality system is numerically solved based on an iterative method.