Background & aim: Urinary tract infection (UTI) is one of the most common bacterial infections, which affected the majority of community. Escherichia coli as a most frequent causative agent of UTI have high resistant rate to antibiotic therapy. The aim of this study was to investigate the prevalence of antimicrobial resistance pattern of E. coli isolated from patients with UTI in Yasuj city. Methods: In the present descriptive study, over a 6 months period, 120 samples of E. coli were collected from patient with UTI and confirmed by biochemical tests. Susceptibility pattern of bacterial isolates against 11 antibiotics carried out by disc diffusion method. Data were analyzed by SPSS software version 16. Results: Among 120 isolated E. coli, the greatest resistance to cephalothin (100%) and the least resistance to imipenem (83/0%) were seen respectively. Susceptibility pattern to other antibiotics is as follow: amoxicillin-clavulanic acid (78.3%), cotrimoxazole (62.5%), tetracycline (55%), nalidixic acid (48.3%), ceftazidime (41.6%), ciprofloxacin (28.3%), gentamicin (20.8%), chloramphenicol (7.5%) and amikacin (5%). Conclusion: It is recommended to treat urinary tract infections by using fewer antibiotics such as Amoxicillin / Clavulanic acid and co-trimoxazole, and administration of ciprofloxacin and gentamicin should be used with caution.