Objective: The purpose of this study was to investigate the effect of eight-weeks general preparation exercise on some selected biomechanical, anthropometrical and physiological parameters of the Iranian national Females' Taekwondo team. Methods: Nine elite members of Iranian national females' Taekwondo team (age 23/22±1/98 years old, and weight 61/88±8/44 kg) participated in this study. Biomechanical, anthropometrical and physiological parameters including; speed, agility, visual reaction time, anaerobic power, body composition, body weight, cardiovascular endurance, muscular endurance. These variables were measured before and after 8 weeks (72 sessions) general exercise training. The Shapiro-Wilk test was used to check the normality of distribution, also using a paired sample t-test to compare variables before and after training with a significant level of (p≤0/05). Results: The results showed that the body fat percentage of subjects significantly decreased (P=0.000).The results of body weight, agility, visual reaction time and anaerobic power (ergo jump test) test were slightly lower after training protocol. The results of anaerobic power (vertical jump) (P=0.01), cardiovascular endurance (P=0.000) and muscle endurance (P=0.001) had been significantly increased. Conclusion: According to the result of this study, it seems that applied training program had the main effect on body composition, cardiovascular endurance, muscle endurance and physical performance of elite taekwondo athletes that will increase the qualitative athletic performance.