The effects of zinc oxide nanorod (ZnO-N) incorporation on thephysicochemical properties of tapioca starch / bovin gelatin composite film such as water absorption capacity (WАC), water solubility and permeability to water vapour (WVP) were investigated. In this search, ZnO-N was homogenized by sonication and added into tapioca starch / bovine gelаtin dispersions аt different concentrations (e.g. 0.5, 2, and 3.5% w/w total solid). Incorporation of 3.5% of nanoparticles to tapioca starch / bovine gelatin films decreased the permeability to water vapor by 18%. Water absorption capacity and Solubility of the films were decreased by increasing the ZnO-N contents. These properties suggest that ZnO-N has the potential as filler in starch /gelatin-based films for using in pharmaceutical and food packaging industries