The aim of our research is to investigate the physical connection between light, perception, and information by means of an integrated methodological approach. The proposed method was used for sustainable lighting design aiming at light efficacy and quality, visual perception, communication, and energy saving. Three types of synergic measurement were carried out: photometric, colourimetric, and perception measurements. Starting from the results and the post-processing of photometric and colourimetric measurements on different light sources and fresco surfaces, by applying the eye-tracking technique for eye movement analysis of a people sample, entropy calculations were carried out. The results of observer perception and preferences, patterns of their visual scanning due to different LED (Light Emitting Diode) sources, were quantified by means of Shannon entropy calculation and, consequently, the information content of images that each light source can transmit. A new lighting proposal, based on the use of LED sources characterized by a spectral emission of light in strong compliance with surface colour and relevant transmission image content information was suggested and checked by transient simulations. Our proposal was based on sustainability because this concept is linked to developing perception and visual wellbeing, information, light communication, and lower energy consumption for maintenance, but especially for preventive conservation and the protection of works of art by means quality of light.