Plant tissue culture tecnique can be used to produce chemical compound or secondary metabolites from medicinal plant. The chemical compound of medicinal plants sometime produced locally within the callus. These compounds are generally identical with the one produced by medicinal plantgrowth in the field.

Sonchus arvensis (tempuyung) used as diuretic and anti-kidney stone. The flavonoid compound of Sonchus arvensis related to such activities are Apigenin 7-glucoside and Luteolin 7-glucoside. The both compounds of Sonchus arvensis are active as anti-kidney stone and diuretic by plant tissue  culture. The reseach was carried out in plants tisue culture laboratory at BPTO Tawangmangu from June to Jully 2002.

The callus of Sonchus arvensis  aged of 50 days was analized with spot test and TLC methode.

The result was compared to the chemical compounds from Sonchus arvensis which growth in the field. The result showed that chemical compound from  the callus and the plant growth in the field were identical.