Background and Objectives: Compliance of hand hygiene among staffs is one of the most important ways of hospital infection control. Existance of a uniform method for the measurement of hand hygiene in hospitals could have a major impact on the prevention of infection. In this study, the rate of hand hygiene in ICU personnel of Kamkar hospital, was investigated.                                                                                                                                                Methods: This study was conducted as a descriptive cross-sectional study on all ICU personnel of Kamkar Education, Research and Treatment Center of Qom in 2014. To collect the data, a checklist sent from the Ministry of Health (recommended by the World Health Organization) was used. Results: In this study, the mean compliance of hand hygiene in ICU was 44.5%, of which the highest percentage was allocated to the nurses. Also, use of alcohol solution for hand rubbing was more than water and soap, and the highest rate of hand hygiene compliance was reported for situations after exposure to blood and patient’s body fluids. The least compliance was related to the situations before contact with patient. Conclusion: According to the results of this study, It is necessary that more trainings be given to improve hand hygiene compliance, some arrangements be made to avoid overwork of personnel, and the equipments needed for this health behavior be provided.