The biodiversity of spider wasps in Psorthapsis occurring in Colombia was examinedin this study. Three species of this genus were found in northern and westernColombia, of which two represent new distributional records: Psorthaspis variegataand Psorthaspis connexa are recorded from Chocó and Magdalena, respectively.A neotype is established for Psorthapsis colombiae. The morphological variation,coupled with geographic distribution, precludes the division of P. variegata and P.connexa into subspecies. A discussion on the status of these subspecies is provided.A key to the members of the genus in Colombia also is presented.