Antibiotic residues pose a threat to the health of aquatic organisms. The effects and accumulation of antibiotic ciprofloxacin (CIP) in a floating macrophyte (Eichhornia crassipes) under hydroponic conditions were investigated. It was found that E. crassipes exposure to CIP (< 1000 μg L) could maintain a stable photosynthesis efficiency. In response to CIP stress, catalase and peroxidase activities of leaves were 7.24-37.51 nmol min g and 98.46-173.16 U g, respectively. The presence of CIP did not inhibit the growth of the plant. After 14 days of exposure, tender leaves became white and withered, ascribed to the decline of chlorophyll content and chlorophyll fluorescence parameters. The CIP concentrations, absorbed by E. crassipes, were highest in the roots, followed by white aerial parts and green aerial parts at CIP concentrations of 100 and 1000 μg L. These findings demonstrated that E. crassipes could absorb and tolerate CIP in a limited time-scale and imply an alternative solution for phytoremediation in water bodies contaminated with antibiotics.