The semi-continuous anaerobic digestion (AD) performances of dry chicken manure (DCM) were investigated at the temperature of 35 ± 1 °C with and without biochar. The average specific methane productions of 0.18 L/g VS and 0.17 L/g VS were achieved without biochar at the organic loading rate (OLR) of 3.125 and 6.25 g VS/L/d, respectively. An increase of 12% in methane production was obtained in the presence of biochar at the two operational OLRs. Accumulation of propionic acid was observed associating with AD of DCM, which was substantially alleviated by biochar supplement. The buffer capacity of biochar was supposed to develop through strengthening the buffer system established by NH and volatile fatty acids. Methanosarcina that can utilize multiple nutrients for methanogenesis was the dominant archaea in the presence of biochar, while the strictly aceticlastic Methanosaeta was dominant in control digester. These results suggest that biochar enhanced methanogenesis through intensifying its available pathway.