This paper applied speech recognition and RFID technologies to develop an omni-directional mobile robot into a robot with voice control and guide introduction functions. For speech recognition, the speech signals were captured by short-time processing. The speaker first recorded the isolated words for the robot to create speech database of specific speakers. After the speech pre-processing of this speech database, the feature parameters of cepstrum and delta-cepstrum were obtained using linear predictive coefficient (LPC). Then, the Hidden Markov Model (HMM) was used for model training of the speech database, and the Viterbi algorithm was used to find an optimal state sequence as the reference sample for speech recognition. The trained reference model was put into the industrial computer on the robot platform, and the user entered the isolated words to be tested. After processing by the same reference model and comparing with previous reference model, the path of the maximum total probability in various models found using the Viterbi algorithm in the recognition was the recognition result. Finally, the speech recognition and RFID systems were achieved in an actual environment to prove its feasibility and stability, and implemented into the omni-directional mobile robot.