Aim. To investigate the effect of Nocardia vaccinii ІMV B-7405 surface-active substances (surfactants) on adhesion of some bacteria and yeasts to fragments urogenital silicone catheters. Methods. The degree of adhesion of microorganisms was determined as the ratio of the cells amount on treated by surfactants catheters fragments to the amount of cells in the control (without surfactant treatment) samples and expressed in percent. Cell concentration was determined by the method of Koch meat-peptone agar and glucose-potato agar. Results. It was shown that the attachment bacteria (Escherichia coli IEM-1, Erwinia sp. TБ-4, Enterobacter cloaceae AС-22, Pseudomonas sp. MI-2, Proteus vulgaris ПA-12) and yeast Candida albicans Д-6 to the fragments of urogenital silicone catheters treated with N. vaccinii IMB B-7405 surfactants depended on the concentration and purification of surfactants, and the type of test cultures. Treatment catheters of N. vaccinii IMB B-7405 surfactant (0.05–0.08 mg/ml) was accompanied by decrease adhesion of bacteria and yeast by 21–95% and 57–94%, respectively. Conclusions. Thus, N. vaccinii ІMB B-7405 surfactant can be used for development of effective preparations decreasing the adhesion of microorganisms on the surface of medical application.