In this study, we determined the accuracy Vicam method for the determination of aflatoxin B1 in feed and aflatoxin M1 in milk and dairy products by applying the method of standard addition. Analytical yield in all causes ranged from 93 to 95%, indicating a high accuracy of the method. The determination was made on aflatoxin B1 in (n = 20) samples of corn and adequately in milk and dairy products taken from animals that were fed the same corn. The samples are taken from small farms Pelagonia region. The results were in the normal range for corn and matches the reference value given approval for accreditation of laboratories in our country. For milk that was not sufficiently investigated field we get results that are in accordance with the Rules of the European Union, the aflatoxin M1 presence to 0.05 ppb. We conclude that the determination of aflatoxin B1 important, their presence in animal feed in order to avoid the presence of aflatoxin M1 in milk and dairy products, and it certainly depends on the season when the use of corn in the diet, time, storage temperature, etc.