The purpose of our study was to conduct a comparative analysis of tuberculosis incidence among healthcare workers in the general medical service departments and anti-tubercular departments in the Dnipropetrovsk region for the period from 2008 to 2017, analysis of the professional, clinical structure of tuberculosis cases for 2015-2017. Despite the decrease in the incidence of tuberculosis inUkraine,  the incidence of morbidity among medical workers in the Dnipropetrovsk region remains unfavorable. Over the past 3 years, 155 employees of health facilities have been infected with tuberculosis, including 134 from the institutions of the general medical profile and 21 employees of the anti-TB clinics. The risk group consists of representatives of scrub staff and nursing staff, as well as auxiliary (other) personnel, regardless of the profile of the medical institution. It was established that the morbidity of the staff of the institutions of the community network and the clinical structure of cases of tuberculosis is unfavorable with a high proportion of destructive changes in the lungs, bacterial discharge, less specific gravity of cases detected during prophylactic examinations.