In this study, the cytotaxonomy of the genus Leucopoa Griseb in Iran was investigated. This genus possesses two speices namely L. sclerophylla and L. pseudosclerophylla in Iran. A total of three accessions belonging to L. sclerophylla and L. pseudosclerophylla were examined in this investigation. So far, no chromosome number has been reported in this genus. The diploid numbers counted in this study were 2n=42 and x=7 for three populations belonging to two studied species. The karyotypic observations and chromosomal parameters showed more or less nonsymmetrical profiles in this genus.