Various methods are been used to control post-harvest diseases of tomato fruits. With the increasing restrictions on the use of fungicides, due the food safety and environmental impacts, the use of alternative methods and biological control of postharvest diseases have been stimulated. The aim of this work was to investigate the effect of alternative products in post-harvest treatment of tomato “Delta” and “Debora” produced under two cropping systems: intermediate (IS) and conventional system (CS). The components evaluated were the reduction of fresh mass, pH, soluble solids (SS), titratable acidity (TA) and the pathogens incidence after being treated by immersion with acibenzolar-S-methyl (ASM), Azadirachta indica crude extract, citric biomass extract and fumigation with essential oils (EO) of Syzygium aromaticum and Citrus aurantium ssp. bergamia. The products did not affect the parameters analyzed in the fruits from the CS, regardless of cultivar. In the fruits produced in IS was observed influence of the treatments. For weight reduction, were observed the lowest values in fruits of “Debora” treated with EO and ASM. Were observed also, the highest values of SS and TA in fruits “Debora” treated with S. aromaticum EO and ASM. The fruits “Delta” had the highest pH when treated with S. aromaticum EO.