A previous study examined, in detail, the morphological response of hybrid Cymbidium Twilight Moon ‘Day Light’ protocorm-like bodies (PLBs) to 26 plant growth regulators (PGRs). In this study, flow cytometric analyses of the PLBs derived from several of these PGR treatments revealed changes in the ploidy of PLBs while the ploidy of plant leaves remained constant. The SPAD value of leaves of plants derived from PGR treatments changed significantly. The choice of PGR must be accompanied by careful scrutiny of the possible resulting changes to morphology and physiological parameters.