Background & aim: Adipokines, including omentin and chemerin, are cytokines that are mainly secreted by adipose tissue. The aim of the present study was to investigate the effect of eight weeks of intense aerobic exercise on chemerin and omentin plasma levels in female Sprague Dawely rats. Methods: In the present experimental study, 35 two-month old Sprague Dawely rats were randomly divided into two groups: control (15 rats) and training (20 rats). The training program included aerobic exercises which were conducted increasingly five times a week for 8 weeks. Blood samples were taken 24 h after the last training session. After eight weeks, an independent t-test and Pearson's correlation coefficient were used for data analysis. Results: The results showed that there was significant difference between omentin serum levels (p=0.012) and chemerin serum levels (p=0.003) in rats that had undergone exercises with high intensity program as compared to the control group. In addition, no significant correlation was observed between omentin and chemerin levels (p=0.08). Conclusion: According to the results of the present study as well as the importance of omentin and chemerin level changes, it seems that the training time must be more than 8 weeks and the exercise intensity should be tailored to the individual's fitness level. Otherwise, subjects are not able to perform the exercise program properly.