The aim of this study was to know the success and differences of pit & fissure sealant materal penetration of composite resin type (UltraSeal XT Plus) into fissure by using syringe blue micro tips and syringe white mini brush tips in fissure closure efforts for caries prevention of permanent teeth. The study was conducted to 30 maxillary first premolar, using syring blue micro tips, and 30 maxilary first premolar using syringw white mini brush tips. The samples, then, were made smears and observed by optic microscope with 300 times magnification. The result of this stuy showed that mean of pit & fissure sealant material penetration of composite resin type into fissure by using blue micro tips was (67.93 ± 13.09)%. By using syringe white mini brush tips was (92.96 ± 6.18)%. With t-test, it showed a significant difference (t = 9.84, p<0,01).